Outbreak of Clostridioides difficile infection in Silesian district hospital
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Department of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland / Katedra i Zakład Mikrobiologii Lekarskiej, Wydział Nauk Medycznych w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
Student Research Group, Department of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland / Studenckie Koło Naukowe przy Katedrze i Zakładzie Mikrobiologii Lekarskiej, Wydział Nauk Medycznych w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
Department of Microbiology, KORLAB Medical Laboratory, Ruda Śląska, Poland / Pracownia Mikrobiologii, KORLAB Laboratoria Medyczne, Ruda Śląska
Corresponding author
Klaudia T. Szarek   

Katedra i Zakład Mikrobiologii Lekarskiej, Wydział Nauk Medycznych w Katowicach, Śląski Uniwersytet Medyczny w Katowicach, ul. Medyków 18, 40-752 Katowice
Ann. Acad. Med. Siles. 2023;77:75–81
In order to evaluate a Clostridioides difficile infection (CDI) outbreak between December 2018 and February 2019 in the internal medicine ward of a district hospital in Silesia, 6 stools from 5 patients were examined.

Material and methods:
C. difficile was identified, genes encoding glutamate dehydrogenase (GDH) – gluD, A/B – tcdA/tcdB and binary – cdtA/cdtB toxins, ermB were determined by mPCR and antibiotic resistance by means of E-Tests.

Women predominated among the patients (4/5). All the 6 C. difficile isolates belonged to hyperepidemic ribotype 027, were positive for all genes and were resistant to moxifloxacin, erythromycin, clindamycin, rifampicin, imipenem, and chloramphenicol.

The obtained results indicate that the hyperepidemic C. difficile clone is spreading in the ward.

The authors gratefully acknowledge Prof. Ed Kuijper and Dr Celine Harmanus (Department Of Microbiology, Leiden University Medical Center, The Netherlands) for the C. difficile ribotyping.
This study was financed by a grant from the Medical University of Silesia no. PCN-1-126/K/0/I.
Undeclared conflict of interest.
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