Determining content of mercury in vitamin and mineral dietary supplements
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Department of Toxicology and Bioanalysis, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice
Apteka Staromiejska, Gliwice
Department for Experimental Medicine, Faculty of Medical Science in Katowice, Medical University of Silesia in Katowice
Barbara Brodziak-Dopierała   

Department of Toxicology and Bioanalysis, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Poland, ul. Jagiellońska 4, 41-200 Sosnowiec
Ann. Acad. Med. Siles. 2019;73:203–211
Dietary supplements are increasingly more frequently used to increase the daily intake of essential vitamins and minerals. These preparations are classified as a special category of foods and are subject to food law, not pharmaceutical regulations. As a result, the requirements for dietary supplements are much less restrictive than to medications. The aim of study was to determine the level of mercury in multivitamin and multimineral preparations and to analyze the results.

Material and methods:
The study included 22 dietary supplements containing vitamins and minerals available on the Polish market. Mercury was analyzed with an AAS using AMA 254.

The concentrations of Hg were 0.22–5.85 µg/kg (the average content was 2.40 µg/kg). The highest average mercury content was 4.51 µg/kg, whereas the lowest one was 0.31 µg/kg. The results were also analyzed in individual groups depending on the main component and indication for use as well as pharmaceutical formulation. Furthermore, according to the manufacturer’s recommendation, the dose of mercury taken with a single medicine was estimated for time of a day, a week, a month, 3 months and also a year. The findings were compared to the limits defined in the regulations of the European Union and PTWI set by the JECFA to assess the health risk. The results presented in this study proved that the amount of mercury in the tested dietary supplements is much lower than the value of valid regulations.

As long as they are used with the recommendation of manufacturer, the analyzed supplements do not pose a threat to human health.

Szewczyk P. Dopuszczanie do obrotu suplementów diety. Najwyższa Izba Kontroli LLO.430.002. Nr ewid. 195/2016/P/16/078/LLO. 2016 [online], [Dostęp: 10.03.2018].
Bojarowicz H., Dźwigulska P. Suplementy diety. Część I. Suplementy diety a leki – porównanie wymagań prawnych. Hygeia Public Health 2012; 47: 427–432.
Prawo suplementów diety. Red. nauk. M. Kondrat. Wolters Kluwer 2012. Warszawa, s. 16–27.
Krejpcio Z., Skwarek K., Hyżyk A., Dyba S. Ocena powszechności spożycia suplementów diety w wybranej grupie osób aktywnych sportowo. Probl. Hig. Epidemiol. 2011; 92(4): 935–938.
Krasnowska G., Sikora T. Suplementy diety a bezpieczeństwo konsumenta. Żywność. Nauka. Technologia. Jakość 2011; 4(77): 5–23.
Ustawa o bezpieczeństwie żywności i żywienia z dnia 25 sierpnia 2006 r. (Dz. U. z 2010 r. Nr 136, poz. 914 z późn. zm.) Zasady wprowadzania po raz pierwszy do obrotu suplementu diety, środków spożywczych specjalnego przeznaczenia żywieniowego oraz żywności wzbogaconej [online], (accessed on 15 December 2018) [Dostęp: 11.03.2018].
Kowalski A., Frankowski M. Levels and potential health risks of mercury in prescription, non-prescription medicines and dietary supplements in Poland. Regul. Toxicol. Pharmacol. 2015; 73(1): 396–400, doi: 10.1016/j.yrtph.2015.08.001.
Genuis S., Schwalfenberg G., Siy A., Rodushkin I. Toxic element contamination of natural health products and pharmaceutical preparations. PLoS One 2012; 7(11): 1–12, doi: 10.1371/journal.pone.0049676.
Bolan S., Kunhikrishnan A., Seshadri B., Choppala G., Naidu R., Bolan N.S., Ok Y.S., Zhang M., Li C.G., Li F., Noller B., Kirkham M.B. Sources, distribution, bioavailability, toxicity, and risk assessment of heavy metal(loid)s in complementary medicines. Environ. Int. 2017; 108: 103–118. doi: 10.1016/j.envint.2017.08.005.
Moret S., Prevarin A., Tubaro F. Levels of creatine, organic contaminations and heavy metals in creatine dietary supplements. Food Chem. 2011; 126: 1232–1238.
WHO (World Health Organization). Mercury and health, 2013. [online], [Dostęp 13.03.2018].
Abernethy D.R., Destefano A., Cecil T., Zaidi K., Williams R.L. Metal impurities in food and drugs. Pharm. Res. 2010; 27(5): 750–755, doi: 10.1007/s11095-010-0080-3.
Genchi G., Sinicropi M., Carocci A., Lauria G., Catalano A. Mercury exposure and heart diseases. Int. J. Environ. Res. Public Health 2017; 14(1): pii: E74, doi: 10.3390/ijerph14010074.
Seppanen K., Kantola M., Laatikainen R. Effect of supplementation with organic selenium on mercury status as measuered by mercury in pubic hair. J. Trace Elem. Med. Biol. 2000; 14(2): 84–89.
Wu X., Cobbina S.J., Mao G., Xu H., Zhang Z., Yang L. A review of toxicity and mechanisms of individual and mixtures of heavy metals in the environment. Environ. Sci. Pollut. Res. Int. 2016; 23(9): 8244–8259, doi 10.1007/s11356-016-6333-x.
Syversen T., Kaur P. The toxicology of mercury and its compound. J. Trace Elem. Med. Biol. 2012; 26(4): 215–226, doi: 10.1016/j.jtemb.2012.02.004.
Bernhoft R. Mercury toxicity and treatment: A review of the literature. J. Environ Public Health 2012; 460508, doi: 10.1155/2012/460508.
Kim M., Lee Y., Park K. Vitamin and mineral supplement use among korean adults: Baseline data from the trace element study of korean adults in Yeungnam area. Nutrients 2018; 10(1): 50, doi: 10.3390/nu10010050.
Park J.H., Hwang M.S., Ko A., Jeong D.H., Kang H.S., Yoon H.J., Hong J.H. Total mercury concentrations in the general Korean population, 2008–2011. Regul. Toxicol. Pharmacol. 2014; 70(3): 681–686, doi: 10.1016/j.yrtph.2014.10.004.
Barrella M.V., Heringer O.A., Cardoso P.M.M., Pimentel E.F., Scherer R., Lenz D., Endringer D.C. Metals content in herbal supplements. Biol. Trace Elem. Res. 2017; 175(2): 488–494, doi: 10.1007/s12011-016-0776-2.
Różycka K., Rolka G. Zastosowanie techniki ASA do oznaczania rtęci na przykładzie badań surowców używanych w przemyśle materiałów budowlanych. Prace Instytutu Ceramiki i Materiałów Budowlanych 2015; 21(8): 58–66.
Raport Najwyższej Izby Kontroli. Informacja o wynikach kontroli. Dopuszczenie do obrotu suplementów diety [online]. Warszawa 2017. [Dostęp 14.03.2018].
Commission staff working document „Characteristics and perspectives of the market for food supplements containing substances other than vitamins and minerals” [online]. Bruksela 5.12.2008 SEC(2008) 2976 [Dostęp 15.03.2018].
Raport PMR. Rynek suplementów diety w Polsce w 2015. Prognozy rozwoju na lata 2015–2020. [Dostęp 15.03.2018].
Europe [Dostęp 15.03.2018].
Herrman J.L., Younes M. Background to the ADI/TDI/PWTI. Regul. Toxicol. Pharmacol. 1999; 30(2Pt2): 109–113.
Socha K., Michalska-Mosiej M. Zawartość rtęci w suplementach diety. Probl. Hig. Epidemiol. 2013; 94(4): 645–647.
Levine K., Levine M., Weber F., Hu Y., Perlmutter J., Grohse P.M. Determination of mercury in an assortment of dietary supplements using an inexpensive combustion atomic absorption spectrometry technique. J. An. Methods Chem. 2005; 4: 211–216, doi: 10.1155/JAMMC.2005.211.
Avula B., Wang Y.H., Duzgoren-Aydin N.S., Khan I.A. Inorganic elemental compositions of commercial multivitamin/mineral dietary supplements: Application of collision/reaction cell inductively coupled-mass spectroscopy. Food Chem. 2011; 127(1): 54–62, doi: 10.1016/j.foodchem.2010.12.083.
Korfali S.I., Hawi T., Mroueh M. Evaluation of heavy metals content in dietary supplements in Lebanon. Chem. Cent. J. 2013; 7: 10, doi: 10.1186/1752-153X-7-10.
Tumir H., Bosnir J., Vedrina-Dragojević I., Dragun Z. Monitoring of metal and metalloid content of dietary supplements on the Croatian market. Food Control 2010; 21(6): 885–889, doi: 10.1016/j.foodcont.2009.12.005.
32. García-Rico L., Leyva-Perez J., Jara-Marini M.E. Content and daily intake of copper, zinc, lead, cadmium and mercury from dietary supplements in Mecixo. Food Chem. Toxicol. 2007; 45(9): 1599–1605, doi: 10.1016/j.fct.2007.02.027.
TNS Raport „Świadome samoleczenie w Polsce – http://www.lekiczysuplementy.p... 2014” z badania przeprowadzonego na zlecenie platformy edukacyjno-informacyjnej http://www.lekiczysuplementy.p... [Dostęp 15.03.2018].
Gershwin M., Borchers A., Keen C., Hendler S., Hagie F., Greenwood M.R. Public safety and dietary supplementation. Ann. N Y Acad. Sci. 2010; 1190: 104–117, doi: 10.1111/j.1749-6632.2009.05270.x.