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Limbal epithelial stem cells in regeneration of corneal epithelium
– clinical and molecular aspects
1
Zakład Biologii Molekularnej, Katedra Biologii Molekularnej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach
2
Oddział Okulistyki Dorosłych, Uniwersyteckie Centrum Kliniczne im. Prof. K. Gibińskiego Śląskiego Uniwersytetu Medycznego w Katowicach, Klinika Okulistyki, Katedra Okulistyki, Wydział Lekarski w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
3
Klinika Okulistyki Katedry Okulistyki, Wydział Lekarski w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
4
Katedra i Zakład Biochemii, Wydział Lekarski w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
5
Stowarzyszenie Śląska Poliklinika Weterynaryjna
6
Uniwersyteckie Centrum Medycyny Weterynaryjnej UJ-UR w Krakowie
Corresponding author
Bartosz Sikora
Zakład Biologii Molekularnej, Katedra Biologii Molekularnej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach, ul. Jedności 8, 41-200 Sosnowiec
Zakład Biologii Molekularnej, Katedra Biologii Molekularnej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach, ul. Jedności 8, 41-200 Sosnowiec
Ann. Acad. Med. Siles. 2018;72:108-115
KEYWORDS
TOPICS
ABSTRACT
Limbal epithelial stem cells (LESC) are located at the junction between the cornea and sclera. Their function is to reconstructing and replace damaged or dysfunctional cells. In this paper information on the characteristics of LESC, describes the methods of their identification using surface molecular markers and also morphological characteristics are included. The methods of LESC cultivation in vitro as a potential source of stem cells for therapy are also presented. The issues of corneal disorders due to limbal stem cell deficiency (LSCD) caused by various factors were described and discussed as well as the treatment methods currently applied in medicine. The standard treatment of LSCD is based on pharmacotherapy and autologous transplantation of the limbus which is rich in stem cells. However, in the case of total stem cell deficiency (TLSCD) allogenic transplantation is necessary.
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