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The influence of rs2273773 and rs7895833 SIRT1 gene polymorphisms on life expectancy in context of metabolic factors in Silesian population
1
Katedra i Klinika Chorób Wewnętrznych, Diabetologii i Nefrologii, Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu, Śląski Uniwersytet Medyczny w Katowicach
Corresponding author
Wladyslaw Jan Grzeszczak
Katedra i Klinika Chorób Wewnętrznych, Diabetologii i Nefrologii, Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu, Śląski Uniwersytet Medyczny w Katowicach
Katedra i Klinika Chorób Wewnętrznych, Diabetologii i Nefrologii, Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu, Śląski Uniwersytet Medyczny w Katowicach
Ann. Acad. Med. Siles. 2017;71:162-172
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Currently, increasingly more genetic variants with an influence on longevity are being sought. One of these is the SIRT1 gene which codes proteins called sirtuins. Regulating transcription, maintaining genomic stability and affecting carbohydrate-lipid metabolism, sirtuins are thought to be longevity enzymes.
Aim of the study:
The aim of the study is to demonstrate the potential relationship between rs2273773 and rs7895833 SIRT1 gene polymorphisms and longevity in the context of metabolic disorders.
Material and methods:
The study encompassed a total of 448 consecutive patients from Southern Poland. The subjects were divided into 2 groups based on age and metabolic disorders. Genotyping of SIRT1 gene polymorphisms was performed using fluorescence-labelled probes and ready-to-use single nucleotide polymorphism determination sets - the TaqMan Pre-designed SNP Genotyping Assay (Applied Biosystems). The Statistica 9.0 program was used for statistical computations.
Results:
In the case of the rs2273773 polymorphism, the prevalence of the TT genotype in the study group was 86.93%, CT 13.07%, CC 0.00%, and TT 91.19%, CT 8.47%, CC 0.34% in the control group. In the case of the rs7895833 polymorphism, the distribution of genotypes in the study group was as follows: AA 67.32%, AG 28.76%, GG 3.92%, and AA 68.47%, AG 28.47% GG 3.05% in the control group.
Conclusions:
No relationship was demonstrated between SIRT1 gene polymorphisms and life expectancy in the Upper Silesian residents.
Currently, increasingly more genetic variants with an influence on longevity are being sought. One of these is the SIRT1 gene which codes proteins called sirtuins. Regulating transcription, maintaining genomic stability and affecting carbohydrate-lipid metabolism, sirtuins are thought to be longevity enzymes.
Aim of the study:
The aim of the study is to demonstrate the potential relationship between rs2273773 and rs7895833 SIRT1 gene polymorphisms and longevity in the context of metabolic disorders.
Material and methods:
The study encompassed a total of 448 consecutive patients from Southern Poland. The subjects were divided into 2 groups based on age and metabolic disorders. Genotyping of SIRT1 gene polymorphisms was performed using fluorescence-labelled probes and ready-to-use single nucleotide polymorphism determination sets - the TaqMan Pre-designed SNP Genotyping Assay (Applied Biosystems). The Statistica 9.0 program was used for statistical computations.
Results:
In the case of the rs2273773 polymorphism, the prevalence of the TT genotype in the study group was 86.93%, CT 13.07%, CC 0.00%, and TT 91.19%, CT 8.47%, CC 0.34% in the control group. In the case of the rs7895833 polymorphism, the distribution of genotypes in the study group was as follows: AA 67.32%, AG 28.76%, GG 3.92%, and AA 68.47%, AG 28.47% GG 3.05% in the control group.
Conclusions:
No relationship was demonstrated between SIRT1 gene polymorphisms and life expectancy in the Upper Silesian residents.
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