Activation energy of viscous flow for some globular and non-globular proteins obtained from viscosity measurements and modified Arrhenius equation
Karol Monkos 1  
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Department of Biophysics, Medical University of Silesia, Zabrze, Poland
Karol Monkos   

Katedra i Zakład Biofizyki SUM, 41-808 Zabrze 8, ul. H. Jordana 19; tel. 32 272 20 41 /236, fax 32 272 01 42
Ann. Acad. Med. Siles. 2009;63:27–38
The aim of the present paper was investigation of the temperature dependence of the activation energy of viscous flow for some proteins in aqueous solutions.

Material and Methods:
The viscosity of hen egg-white lysozyme, bovine β-lactoglobulin, and human, bovine and porcine IgG immunoglobulin aqueous solutions was measured at temperatures ranging from 5oC to 55oC and in a wide range of concentrations. The measurements were performed with an Ubbelohdetype capillary microviscometer.

The average value of the activation energy of viscous flow ΔE can be experimentally obtained from the slope of the line that represents the dependence of the liquid viscosity η (in logarithmic scale) versus a reciprocal of the absolute temperature (T-1). The functional dependence of ΔE on temperature can be obtained from strict definition ΔE = R[dlnη/d(T-1)], where R is the gas constant and from a three parameters modified Arrhenius formula which gives an analytical function describing the viscosity-temperature dependence for proteins solutions in a wide range of temperatures. As appears, ΔE obtained in such a way decreases with increasing temperature according to a square function. The parameters of this function have been obtained for all studied proteins.

The obtained results show that square function describes the temperature dependence of ΔE both for water, solutions and proteins themselves. One of the main factor which influence the activation energy is a molecular mass of protein. However, the results obtained for the studied immunoglobulins IgG show that this factor is not the only one.

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