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2016 vol. 70
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Age-related alterations in arginase-NO-synthase system in patients with coronary heart disease associated with hypertension
Anna Besedina 1
 
 
 
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Department of family medicine FPGE, Lviv National Medical University of Ukraine
 
 
Corresponding author
Anna Besedina   

Department of family medicine FPGE, Lviv National Medical University of Ukraine, 79059, Liviv Emergency Hospital (KMK LSHMD), Mykolaychuka 9, Liviv, Ukraine
 
 
Ann. Acad. Med. Siles. 2016;70:40-45
DOI: https://doi.org/10.18794/aams/43460
 
 
Article (PDF)
References (31)
 
KEYWORDS
coronary heart disease
hypertension
nitric oxide
NO-synthase
arginase
 
TOPICS
Biochemistry
 
ABSTRACT
Introduction:
Coronary heart disease (CHD) is the most common heart diseases in Europe. The aim of this study was to determine the intensity of L-arginine metabolism by two alternative pathways (oxidative by NOS and nonoxid-ative by arginase) in the blood plasma of patients with CHD associated with hypertension (HT) of different age groups.

Materials and methods:
50 patients with isolated CHD and 42 patients with CHD associated with HT were enrolled in this study. NOS activity was determined by nitrite anion formed in the reaction. Arginase activity was tested by the formation of urea.

Results:
In middle-aged patients with isolated CHD, the total NOS activity statistically significantly increased by 2.2 fold in comparison with healthy subjects of the same age group. In patients with CHD associated with HT, the total NOS activity statistically significantly increased in both middle-aged and older persons 2.3-fold than in healthy subjects of the same age groups. In patients with isolated CHD, the arginase activity increases 1.5-fold in middle-aged patients and 1.7-fold in older patients compared with the healthy participants. In the middle-aged and older patients with CHD associated with HT, the arginase activity statistically significantly increased 1.7- and 1.8-fold than in the healthy subjects of the same age groups.

Conclusions:
In patients with isolated CHD and patients with CHD associated with HT, an increased total NO-synthase and arginase activity in comparison with healthy individuals was found. It was shown that the increase in NO-synthase and arginase activity is more expressed in older patients than middle-aged patients.

 
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