Influence of histamine H3 receptors on pressor effect of leptin administered centrally in haemorrhage-shocked rats
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Department of Basic Medical Sciences, School of Public Health in Bytom, Medical University of Silesia
Jerzy Jochem   

Katedra i Zakład Podstawowych Nauk Medycznych Wydziału Zdrowia Publicznego Śląskiego Uniwersytetu Medycznego w Katowicach, ul. Piekarska 18, 41-902 Bytom, tel. +48 600 246 536
Ann. Acad. Med. Siles. 2015;69:132–137
Leptin administered intracerebroventricularly (icv) evokes the resuscitating effect in haemorrhage-shocked rats, and the action is associated with activation of the histaminergic system. The aim of the present study was to examine the possible role of the histamine H3 receptor in leptin-evoked cardiovascular effects.

Materials and methods:
Studies were performed in male Wistar rats subjected to irreversible haemorrhagic shock (0% survival at 2 h) with a mean arterial pressure (MAP) of 20–25 mmHg that were anaesthetized with ketamine/xylazine (100 mg/kg + 10 mg/kg, intraperitoneally). At 5 minutes of critical hypotension the animals were injected icv with leptin (20 µg) or 0.9% NaCl (5 µl).

Haemorrhage resulted in a decrease in pulse pressure (PP) and heart rate (HR). Leptin evoked long-lasting rises in MAP, PP and HR with 100% survival at 2 h. Pre-treatment with histamine H3 receptor inverse agonist/H4 partial agonist clobenpropit (42.5 nmol, icv), but not with H3 receptor neutral antagonist VUF 5681 (50 nmol, icv), inhibited MAP and PP changes evoked by leptin, however, without an influence on HR or survival rate at 2 h.

The histamine H3 receptor can influence the centrally-acting leptin-induced resuscitating effect in haemorrhagic shock in rats.

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